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KMID : 1171820050200010023
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Journal of Environmental and Sanitary Engineering
2005 Volume.20 No. 1 p.23 ~ p.31
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Isolation and Identification of Pathogenic Bacteria from Salads of Fast Food Restaurants
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Park Yong-Bae
Kang Jeong-Bok
Kim Jung-Beom
Kim Jong-Chan
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Abstract
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On the purpose of epidemiological survey relate to food poisoning, a total of 114 samples of different salads collected from fast food Restaurants in Gyeonggi-do were for the presence of pathogenic microorganisms. Microbial assessment of salads revealed that TPC(l.1×10~8.4×10^5 CFU/g) and coliforms(O~5.4×10₄ CFU/g) exceeded the standards by Solberg et al.(TPC:10^5 CFU/g, coliforms:10² CFU/g). Two pathogenic bacteria were isolated from salad samples, and identified by biochemical methods, including API identification systems. Isolates from PALCAM agar and MYP agar media were in 98.6, 99.8% agreements with Listeria monocytogenes, Bacillus cereus at the species level, respectively. All 7 strains of Bacillus cereus isolates produced enterotoxin as revealed with CRET-RPLA,
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KEYWORD
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salad, pathogenic bacteria, identification, CRET-RPLA
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